DOCUMENTATION OF WEB APPLICATION

GitHub Repository for this Web-Application:

Screenshot explanation with radio-number marked picture:

Server Guide Image

Explanation of the marked points:

[1] Selection menu for browsing the pages of the web-server. [2] Peptide/protein submission block for generation of pR value. [3] Submission of virus and protein name for rendering graph in the output box (no.4). [4] Plot generated from virus and protein submission section (no.3). [5] Protein segment variability curve in blue (9aa stretch) & it's axis values are indicated on right side Y-axis. Predicted and scaled B-Cell epitopic property score (for peptide stretch 9aa) in purple line. Predicted and scaled T-Cell epitopic property score (for peptide stretch, 9aa) in cyan line. Predicted and scaled relative solvent accessibility for peptide stretch (9aa) in green line. Solvent accessibility and epitopic properties are scaled within zero to 1 range, with an exception for higher T-Cell values (above 1). Predicted top-20 co-evolving residues marked as black low columns. [6] Scrollbar to scroll through the sequence.

Details of the sequence database:

Organism Name Protein Name Number of Sequences Reference Sequence ID Database Details
SARS CoV 2 Surface Glycoprotein S 20173 YP_009724390 NCBI Virus Database [link]
SARS CoV 2 Envelope Protein 20313 YP_009724392 NCBI Virus Database [link]
SARS CoV 2 Membrane Glycoprotein M 20245 YP_009724393 NCBI Virus Database [link]
SARS CoV 2 ORF3a Protein 20342 YP_009724391 NCBI Virus Database [link]
SARS CoV 2 ORF8 Protein 20237 YP_009724396 NCBI Virus Database [link]
H5N1 Neuraminidase Protein 2943 YP_308668 NCBI Virus Database [link]
H5N1 Hemagglutinin Protein 3669 YP_308669 NCBI Virus Database [link]
H5N1 Matrix Protein M1 3054 YP_308671 NCBI Virus Database [link]
H5N1 Matrix Protein M2 2430 YP_308670 NCBI Virus Database [link]
H1N1 Neuraminidase Protein 23179 NP_040981 NCBI Virus Database [link]
H1N1 Hemagglutinin Protein 22901 NP_040980 NCBI Virus Database [link]
H1N1 Matrix Protein M1 18429 NP_040978 NCBI Virus Database [link]
H1N1 Matrix Protein M2 18088 NP_040979 NCBI Virus Database [link]
H2N2 Neuraminidase Protein 235 YP_308872 NCBI Virus Database [link]
H2N2 Hemagglutinin Protein 204 YP_308850 NCBI Virus Database [link]
H2N2 Matrix Protein M1 226 YP_308854 NCBI Virus Database [link]
H2N2 Matrix Protein M2 229 YP_308853 NCBI Virus Database [link]
H3N2 Neuraminidase Protein 19307 YP_308842 NCBI Virus Database [link]
H3N2 Hemagglutinin Protein 22919 YP_308839 NCBI Virus Database [link]
H3N2 Matrix Protein M1 19486 YP_308841 NCBI Virus Database [link]
H3N2 Matrix Protein M1 18983 YP_308840 NCBI Virus Database [link]
MERS Surface Glycoprotein S 832 YP_009047204 NCBI Virus Database [link]
MERS Envelope Protein E 706 YP_009047209 NCBI Virus Database [link]
MERS Membrane Glycoprotein M 697 YP_009047210 NCBI Virus Database [link]
SARS Surface Glycoprotein S 103472 YP_009724390 NCBI Virus Database [link]
SARS Envelope Protein E 145473 YP_009724392 NCBI Virus Database [link]
SARS Membrane Glycoprotein M 143694 YP_009724393 NCBI Virus Database [link]
SARS Protein ORF3a 273952 YP_009724391 NCBI Virus Database [link]
SARS Protein ORF8 243404 YP_009724396 NCBI Virus Database [link]